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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has become noticed in several tumors, which results in chromosome instability, and so may perhaps add to tumorigenesis. This protein was discovered to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital job in advertising mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, happen to be explained. Two supplemental splice variants were noted but comprehensive nucleotide sequence data is not really yet readily available.

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Blocking peptides are peptides that bind specifically for the target antibody and block antibody binding. These peptide generally consists of the epitope regarded by the antibody.

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This information outlines additional doable optimization of mobile and immunoassay parameters to make sure the very best outcomes are...

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This guidebook outlines further probable optimization of cellular and immunoassay parameters to make certain the very best success are...

Antibodies bound to the blocking peptide not bind to your epitope on the goal protein. By comparing the staining from your blocked antibody versus the antibody on your own, one can see which staining is certain.

Focus on Facts Cyclin E belongs to your really conserved cyclin relatives, whose customers are characterised by a spectacular periodicity in protein abundance with the cell cycle. Cyclins function as regulators of CDK kinases. Various cyclins exhibit distinctive expression and degradation patterns which contribute to your temporal coordination of each and every mitotic function. Cyclin E sorts a posh with and functions as a regulatory subunit of CDK2, whose exercise is necessary for mobile cycle G1/S transition. This protein accumulates in the G1-S phase boundary and is degraded as cells development as a result of S section.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...

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